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anti serca2 (Novus Biologicals)

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Novus Biologicals anti serca2
Anti Serca2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+serca2/SERCA2+ATPase+Antibody+(2A7-A1)/pm41896216-54-63-65
Average 94 stars, based on 10 article reviews

anti serca2 - by Bioz Stars, 2026-11

94/100 stars

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Article Title: LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2
Article Snippet: .. Western blot analysis was performed as previously described.66 The primary antibodies used were anti-FLAG (AF519, Beyotime, 1:1,000), anti-MYOD (ABP53067, Abbkine, 1:500), anti-MyHC (B103, DHSB, 0.5 mg/mL), anti-SERCA2 (NB100-237, Novus, 1:2,000), anti-ACC (PA5-17564, Thermo Fisher Scientific, 1:1,000), anti-pACCSer80 (orb315750, Biorbyt, 1:500), anti-CPT1 (bs23779R, Bioss, 1:500), anti-pmTORSer2488 (#5536, CST, 1:1,000), anti-mTOR (bs-1992R, Bioss, 1:500), anti-ULK1 (bs-3602R, Bioss, 1:500), anti-LC3B (NB100-2220, Novus, 2.0 mg/mL), anti-P62 (18420-1-AP, Proteintech, 1:1,000), anti-CREB (bs-0035R, Bioss, 1:500), anti-pCREBSer133 (ab32096, Abcam, 1:5,000), anti-AMPK (bs-1115R, Bioss, 1:500), anti-pAMPKser712 (ABN-PAB12602, Abnova, 1:2,000), anti-Calcineurin (#2614S, CST, 1:1,000), antiPGC-1a (66369-1-Ig, Proteintech, 1:5,000), and anti-GAPDH (60004-1-Ig, Proteintech, 1:5,000). .. ProteinFind goat anti-mouse IgG(H+L), HRP conjugate (HS201-01, TransGen, 1:1,000) and ProteinFind goat anti-rabbit IgG(H+L), HRP conjugate (HS101-01, TransGen, 1:500) were used as secondary antibodies. mtDNA content and FAO rate assay Total DNA was extracted using a Tissue DNA Kit (D3396, Omega Biotek, USA) following the manufacturer’s protocol.

Incubation:

Article Title: UPR/ATF4/Noxa pathway overactivation through SERCA2 inhibition or ONC201 treatment combined with ABT-737 triggers apoptosis in chemoresistant ovarian cancer cells and patient-derived tumor organoids.
Article Snippet: Immunoblotting: equal amounts of proteins were loaded on 4-15% SDS-PAGE precast gel and transferred onto a PDVF membrane (Bio-Rad). .. Membranes were blocked 1 h with 5% nonfat dry milk in 0.05% Tween-TBS 1X then incubated with the following primary antibodies: anti-ATF4 (#11815), anti-BAK (#3814), anti-BAX (#2774), anti-BCL-xL (#2764), anti-BIM (#2819), anti-BiP (#3177), anti-CASPASE 3 (#9662), anti-CASPASE 8 (#9496), anti-CHOP (#2895), antiDR5 (#8074), anti-MCL-1 (#5453), anti-PARP (#9542), anti-PUMA (#12450) (Cell Signaling Technology); anti-Noxa (#114C307) (Calbiochem); anti-Actin (#MAB1501) (Merck Millipore); antiTubulin (#T6199-100UL) (DM-labo) and anti-SERCA2 (#NB300-581) (Novus-Bio). .. After incubation with the appropriate horseradish peroxidase-conjugate anti-mouse (#NA931V) (Amersham) or anti-rabbit (#7074) (Cell Signaling Technology), revelation was performed using AR TI CL E IN P RE SS ARTICLE IN PRESS Clarity Western EleCtroLuminescence detection reagent (Bio-Rad) and signal was recorded using ImageQuant800 (Cytiva).

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